Live Cell Imaging With Spatial Light Modulator-based Optical Sectioning Structured Illumination Microscopy
نویسندگان
چکیده
Structured illumination microscopy (SIM) is a method in fluorescence microscopy which works by acquiring a set of images using widefield detection. Each image in the set is made with a different position of an illumination mask, but with no mask in the detection path [1]. Subsequent image processing is used to produce an optically sectioned image (OS-SIM) [2 4], or an image with resolution beyond the diffraction limit (super-resolution SIM or SR-SIM) [5,6].
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